Gazprom, Russia's gas monopoly, says it will cut supplies of gas to Ukraine by a further 25% from 1700GMT.
It says shipments are being cut because Ukraine has failed to resolve a dispute over non-payment of debts.
Gazprom Gazprom cuts Ukraine gas supply ...
Ukrainians to work easier in Czech Republic under project ...
Five Czechs die in a small plane's crash in Ukraine ... reduced supplies by 25% from 0700GMT on Monday after talks broke down last week. Ukraine said the reduction was, in fact, 35%.
The state-owned gas company said shipments to Europe through its pipelines were unaffected.
"Twenty four hours have passed since Gazprom reduced supplies by a quarter, however the Ukrainian side has not resumed negotiations and the head of Naftogaz did not arrive in Moscow," Gazprom spokesman Sergei Kupriyanov said.
He said Ukraine still owed money for gas already delivered, but Ukraine maintains the payments have already been made.
Push for talks
Gazprom claims the outstanding debt is $1.5bn (Ј770m) and called on Ukraine to get back around the negotiating table.
Mr Kupriyanov reassured European gas users that shipments of Russian gas, which use pipelines that cross Ukraine, will continue.
"Export deliveries via Ukrainian territory are carried out in full volume," he said.
The Gazprom spokesperson said Ukraine had refused access to two observers hired by Gazprom to monitor gas transit to Europe.
But the Ukrainian energy company Naftohaz Ukrayiny said Gazprom representatives were already present in the country, and that the two observers did not comply with "necessary regulations".
"Such statements are just the next step in escalating tension," Naftohaz Ukrayiny spokesman Valentyn Zemlyanskyy said.
(BBC)
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